MitoVir
Understanding how viruses alter mitochondria to develop novel antivirals
A Brief Introduction
At the Rafie Lab, we’re uncovering how viruses manipulate mitochondria—the powerhouses of the cell—to fuel their replication. By unlocking these secrets, we aim to discover groundbreaking targets for antiviral therapies. Our spotlight is on DNA viruses, like human adenoviruses and herpes viruses, which disrupt mitochondrial roles in immunity, metabolism, and even their structure.
To tackle this challenge, we blend cutting-edge tools from structural biology—like macromolecular x-ray crystallography, single-particle cryogenic electron microscopy, and in situ cryogenic electron tomography—with techniques from biochemistry, biophysics, cell biology, and virology. A key part of our work involves producing viral and host proteins in specialized systems, analyzing them in-depth, and using this knowledge to pinpoint potential antiviral compounds. From there, we guide the design of highly effective antiviral drugs.
Beyond drug discovery, we explore how viral agents hijack host cell processes by visualizing their impact in living cells through fluorescence microscopy. For closer looks at the action, we dive into the molecular details using advanced cryogenic electron tomography. This holistic approach positions us at the forefront of crafting the next generation of antiviral treatments.
